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Korean J Urol Oncol > Volume 2(2); 2004 > Article
The Korean Journal of Urological Oncology 2004;2(2): 55-59.
전립선암세포주에서 Granulocyte Macrophage Colony-Stimulating Factor에 의한 MMP-7, MMP-13의 증가
김태형, 명순철, 김영선, 강군현, 임인자, 방효원
중앙대학교 의과대학 비뇨기과학교실, 1생리학교실
The Granulocyte Macrophage Colony-Stimulating Factor Increase MMP-7 and MMP-9 in Prostate Cancer Cells
Tae Hyoung Kim , Soon Chul Myung , Young Sun Kim , Goon Hyun Kang , In Ja Lim , Hyo Weon Bang
Departments of Urology and 1Physiology, College of Medicine, Chung-Ang University, Seoul, Korea
Published online: June 30, 2004.
Matrix metalloproteinases(MMPs) and tissue inhibitors of metalloproteinases(TIMPs) play role in the invasion and metastasis of prostate cancer, but the mechanism of their regulation is not clearly understood. Recently granulocyte macrophage colony-stimulating factor(GM-CSF) has been shown to be associated with cancer invasion and metastasis. To date, little work has been performed to characterize GM-CSF and MMPs/TIMPs expression in prostate cancer cells. The aim of this study is to evaluate the effects of GM-CSF on production of MMPs/TIMPs in the prostate cancer cells.
Materials and Methods:
Two prostate cancer cell-lines, PC-3 and DU-145 cells were used in this study. Reverse transcription polymerase chain reaction(RT-PCR) was performd to detect the mRNA expression of hGM-CSF. Also, RT-PCR was done to evaluate the mRNA expression level of MMP-2, MMP-7, MMP-9, MMP-13, TIMP-1 and TIMP-2 after being treated with 50ng/ml hGM-CSF.
hGM-CSF were expressed in both cell lines. After hGM-CSF treatment, MMP-7 mRNA expression is increased after hGM-CSF treatment in PC-3 cells. MMP-13 mRNA expression is increased until 12 hours, but decreased at 24, 48 hours after hGM-CSF treatment in PC-3 cells.
These results identify GM-CSF as a regulator of MMP-7 and MMP-13 expression in certain types of prostate cancer cells, and suggest that GM-CSF may contribute to the invasiveness of prostate cancer cells through the regulation of MMP-7 and MMP-13 expression. (Korean J Uro-Oncol 2004;2:55-59)
No abstract available
Key Words: Prostate cancer cell; GM-CSF; Matrix metalloproteinases
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