| Home | E-Submission | Sitemap | Editorial Office |  
top_img
Korean J Urol Oncol > Volume 2(2); 2004 > Article
The Korean Journal of Urological Oncology 2004;2(2): 55-59.
전립선암세포주에서 Granulocyte Macrophage Colony-Stimulating Factor에 의한 MMP-7, MMP-13의 증가
김태형, 명순철, 김영선, 강군현, 임인자, 방효원
중앙대학교 의과대학 비뇨기과학교실, 1생리학교실
The Granulocyte Macrophage Colony-Stimulating Factor Increase MMP-7 and MMP-9 in Prostate Cancer Cells
Tae Hyoung Kim , Soon Chul Myung , Young Sun Kim , Goon Hyun Kang , In Ja Lim , Hyo Weon Bang
Departments of Urology and 1Physiology, College of Medicine, Chung-Ang University, Seoul, Korea
Published online: June 30, 2004.
ABSTRACT
Purpose:
Matrix metalloproteinases(MMPs) and tissue inhibitors of metalloproteinases(TIMPs) play role in the invasion and metastasis of prostate cancer, but the mechanism of their regulation is not clearly understood. Recently granulocyte macrophage colony-stimulating factor(GM-CSF) has been shown to be associated with cancer invasion and metastasis. To date, little work has been performed to characterize GM-CSF and MMPs/TIMPs expression in prostate cancer cells. The aim of this study is to evaluate the effects of GM-CSF on production of MMPs/TIMPs in the prostate cancer cells.
Materials and Methods:
Two prostate cancer cell-lines, PC-3 and DU-145 cells were used in this study. Reverse transcription polymerase chain reaction(RT-PCR) was performd to detect the mRNA expression of hGM-CSF. Also, RT-PCR was done to evaluate the mRNA expression level of MMP-2, MMP-7, MMP-9, MMP-13, TIMP-1 and TIMP-2 after being treated with 50ng/ml hGM-CSF.
Results:
hGM-CSF were expressed in both cell lines. After hGM-CSF treatment, MMP-7 mRNA expression is increased after hGM-CSF treatment in PC-3 cells. MMP-13 mRNA expression is increased until 12 hours, but decreased at 24, 48 hours after hGM-CSF treatment in PC-3 cells.
Conclusions:
These results identify GM-CSF as a regulator of MMP-7 and MMP-13 expression in certain types of prostate cancer cells, and suggest that GM-CSF may contribute to the invasiveness of prostate cancer cells through the regulation of MMP-7 and MMP-13 expression. (Korean J Uro-Oncol 2004;2:55-59)
ABSTRACT
No abstract available
Key Words: Prostate cancer cell; GM-CSF; Matrix metalloproteinases
TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
CrossRef TDM  CrossRef TDM
  E-Mail
Share:      
METRICS
305
View
7
Download
Related article
Editorial Office
Department of Urology, Chung-Ang University Hospital
102 Heukseok-ro, Dongjak-gu, Seoul 06973, Korea
Tel: +82-2-6299-1819   Fax: +82-2-6294-1406   E-mail: journal@kjuo.or.kr
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © The Korean Urological Oncology Society and The Korean Prostate Society. All rights reserved.                 developed in m2community
Close layer
prev next