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Korean J Urol Oncol > Volume 10(2); 2012 > Article
The Korean Journal of Urological Oncology 2012;10(2): 41-49.
식물 및 곤충세포 발현시스템을 이용한 암 진단 및 치료용 항체 생산
이정환, 김득수, 이재혁, 명순철, 황경아1, 추영국2, 고기성
중앙대학교 의과대학 의학부, 1국립농업과학원 농식품자원부, 2원광대학교 자연과학대학 생명과학부
Production of Cancer Diagnostic and Therapeutic mAb Using Plant and Insect Expression Systems
Jeong-Hwan Lee , Deuk-Su Kim , Jaehyouk Lee , Soon-Chul Myung , Kyung-A Hwang 1, Young-Kug Choo 2, Kisung Ko
Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University, Seoul, 1Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Suwon, 2Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Korea
Published online: August 30, 2012.
ABSTRACT
Therapeutic proteins can prevent or treat wide ranges of diseases from cancer and viral or bacterial infections. Production of the therapeutic proteins has been well established mainly in mammalian and bacterial cells including transgenic organisms by recombinant DNA techniques. Recent plant and insect biotechnology and advanced molecular immunology have established molecular biofarming system as an alternative way to produce recombinant pharmaceutical proteins such as immunotherapeutic monoclonal antibodies (mAb). The plants and insects as an expression system have several advantages, which include the lack of animal pathogenic contaminants, low cost of production, and ease of agricultural scale-up compared to other currently available systems. Thus, the paradigm of plant is being shifted as a food source to so-called plant bioreactor for production of therapeutic proteins. Currently, we have successfully developed a plant and insect cell expression system for production of anti-cancer and anti-virus monoclonal antibodies. The effective heterologous production system for recombinant therapeutics requires the appropriate expression machinery with optimal combination of transgene expression regulatory conditions such as control of transcriptional and post transcriptional events. In this paper, the possibility of targeting proteins to the ER and possibly storing them to the protein storage vacuoles with glycosylation modification are discussed in plant and insect cells. (Korean J Urol Oncol 2012;10:41-49)
Key Words: Plant; Insect cell; Therapeutic proteins
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