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Korean J Urol Oncol > Volume 8(3); 2010 > Article
The Korean Journal of Urological Oncology 2010;8(3): 122-127.
전립선암에서 인체신경줄기세포를 이용한 Cystosine Deaminase 자살유전자 세포치료법 개발: Cystosine Deaminase 자살유전자도입 인체신경줄기세포의 전립선암으로 회귀
송윤섭, 이홍준1,2, 두승환,2, 양원재2, 김대홍3, 이상진4, 김승업1,2
순천향대학교 의과대학 비뇨기과학교실, 1중앙대학교 의과대학 의학연구소, 2British Columbia 대학교 의과대학 신경과, 국립암센터 3분자영상치료연구과, 4비뇨생식기암연구과
Migration Ability of Cytosine Deaminase Gene Expressing Human Neural Stem Cells Toward Prostate Cancer
Yun Seob Song , Hong Jun Lee 1,2, Seung Whan Doo ,2, Won Jae Yang 2, Dae Hong Kim 3, Sang Jin Lee 4, Seung Up Kim 1,2
Department of Urology, Soonchunhyang University School of Medicine, 1Medical Research Institute, Chungang University School of Medicine, Seoul, Korea, 2Division of Neurology, Department of Medicine, University of British Columbia, Vancouver, Canada, 3Department of Molecular Image Therapy Research, 4Urologic Cancer Research, National Cancer Center, Ilsan, Korea
Published online: December 1, 2010.
ABSTRACT
Purpose:
Stem cells have migration ability toward cancer cells and therapeutic genes can be easily transduced into stem cells with viral vectors. Cystosine deaminase (CD) is the enzyme that transforms the prodrug 5- fluorocytosine to highly toxic drug 5-fluorouracil (5-FU). This study was performed to investigate the migration ability of CD gene transduced human neural stem cells (Hb1.F3-CD) toward prostate cancer after systemic treatment in C57B mice.
Materials and Methods:
An Hb1.F3 with a retroviral vector encoding v-myc, lacZ was generated and transfected with recombinant retrovirus from the pMSCV-puro/CD. Reverse transcription (RT) was performed to evaluate the expression of CD in Hb1.F3-CD. In vivo migration assay after systemic injection of Hb1.F3-CD was performed in prostate cancer induced C57B mice using molecular magnetic resonance (MR) imaging and immunochemical staining.
Results:
Transgene expression in CD-hNSCs was demonstrated. In vivo migration ability of CD-hNSCs cells toward TRAMPC2 induced prostate cancer was found using MR imaging and X-gal stain after implantation of HB1.F3-CD in C57B mice.
Conclusions:
We confirmed the migration ability of HB1.F3-CD toward prostate cancer cells after systemic treatment in C57B mice. This finding may imply a possible novel selective chemotherapeutic strategy against prostate cancer. (Korean J Urol Oncol 2010;8:122-127)
Key Words: Stem cell; Prostate cancer; Migration; Gene; Cytosine deaminase
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