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Korean J Urol Oncol > Volume 8(1); 2010 > Article
The Korean Journal of Urological Oncology 2010;8(1): 27-31.
OCT Compound로 포매한 전립선 동결절편에서 RNA 추출방법 비교
전성윤, 이형남, 김태정, 정은선, 이교영, 최영진
가톨릭대학교 서울성모병원 병리과
Evaluation of Total RNA Extraction Methods for OCT Compound -Embedded Frozen Prostate Tissue Sections
Sung Yoon Jeon , Hyoungnam Lee , Tae-Jung Kim , Eun Sun Jung , Kyo Young Lee , Yeong-Jin Choi
Department of Pathology, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea
Published online: April 1, 2010.
ABSTRACT
Purpose:
There are two methods commonly used for the extraction of total RNA. One is a Trizol method and the other is a column method. However, there have been few reports comparing the quality of total RNA extracted by these two methods in fresh frozen OCT compound-embedded (FFOE) tissues. We evaluate the two RNA extraction methods, to know the best quality of total RNA in FFOE tissues.
Materials and Methods:
Twenty- one fresh frozen human prostate tissues were used for RNA extraction by the classic method using Trizol reagent and the commercially available method, respectively. RNA purity and quality analysis were performed by spectrophotometry and automated electrophoresis system.
Results:
An A260:A280 ratio of all RNA from two methods were above of 1.8. The ratio of 28S/18S rRNA in Trizol method (tumor=0.93±0.25, nontumor=1.03±0.28) were higher than the column method (tumor=0.58±0.20, nontumor= 0.60±0.28, p<0.001). On gel images, 18S and 28S rRNA were separated and intact in Trizol method but they were not visible as intact bands but fragmented in column method.
Conclusions:
To purify the best quality RNA from the FFOE tissues for the molecular pathologic study, Trizol method is superior to the column method. (Korean J Urol Oncol 2010;8:27-31)
Key Words: RNA; Frozen sections; Prostate; Method
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